Fig. 4. Lysosomal degradation pathway. HEK-293 cell lines stably expressing (A) A1038V, or (B) G1961E were treated for 16 h with increasing doses of a proton pump inhibitor of lysosome degradation, bafilomycin A. Data are normalized to the 0 µM control. *P<0.05. n=3-4. Ezrin was used as the loading control. The A1038V mutant is degraded in the lysosome, but G1961E is not.